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Handbook of Cryo-Preparation Methods for Electron Microscopy (Methods in Visualization) ebook

by Annie Cavalier,Daniele Spehner,Bruno M. Humbel


Using transmission electron microscopy we have explored the toxicity action of various doses of cyclosporin A (CyA) on testes and adrenals of rats

Annie Cavalier, Danielle Spehner, and Bruno M. Humbel (Ed. Handbook of Cryo-Preparation Methods for Electron Microscopy. CavalierAnnie, SpehnerDanielle, and HumbelBruno M. (Ed. Using transmission electron microscopy we have explored the toxicity action of various doses of cyclosporin A (CyA) on testes and adrenals of rats. The result is that the development of the cells of seminiferous is blocked in CyA 10 mg/kg group. CyA 20 mg/kg can destroy the cell structures of the seminiferous and induce the cell denaturation of zones of adrenal cortex.

Danièle Spehner The invention of cryo-electron microscopy, which allows bacterial cells to be imaged in a frozen-hydrated, near-native state without the need fo. .

Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. The invention of cryo-electron microscopy, which allows bacterial cells to be imaged in a frozen-hydrated, near-native state without the need for dehydration and stains, has now led to important new insights. Efforts to identify structures and localize specific proteins in cryo-EM images are summarized.

Start by marking Handbook of Cryo-Preparation Methods for Electron .

Start by marking Handbook of Cryo-Preparation Methods for Electron Microscopy as Want to Read: Want to Read savin. ant to Read. O Describe cryo-methods aimed at perfect preservation for fine structural analysis. O Teach how to arrest physiological processes by cryo-fixation. O Reveal the secrets for high-resolution snapshots of life by CEMOVIS. O Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography. O Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules.

Annie Cavalier, Daniele Spehner, Bruno M. Humbel.

Cellular Electron Microscopy (Methods in Cell Biology, Vol. 79). San Diego, CA: Academic Press. Low Temperature Methods in Biological Electron Microscopy (Practical Methods in Electron Microscopy). Recommend this journal.

Handbook of Cryo-Preparation Methods for Electron Microscopy (Methods in Visualization). Annie Cavalier, Daniele Spehner, Bruno M. Category: Техника, Электроника. 24. 8 Mb. In Situ Hybridization in Electron Microscopy (Methods in Visualization). Gerard Morel (Author), Annie Cavalier (Author). 1. 6 Mb. In Situ Hybridization in Light Microscopy (Methods in Visualization). Gerard Morel, Annie Cavalier.

Download Handbook of Cryo Preparation Methods for Electron Microscopy Methods in Visualization Book.

Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography

Print publication date: October 2008 Online publication date: October 2008. Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography. Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules. Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography.

While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.

The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.

Contributions to this handbook –

Ö Describe cryo-methods aimed at perfect preservation for fine structural analysis

Ö Teach how to arrest physiological processes by cryo-fixation

Ö Reveal the secrets for high-resolution snapshots of life by CEMOVIS

Ö Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography

Ö Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules

Ö Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography

Ö Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding

Ö Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling

Ö Include summary tables to compare and obtain appropriate criteria

Includes a wealth of immediate reference material for daily use

With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.

Handbook of Cryo-Preparation Methods for Electron Microscopy (Methods in Visualization) ebook
Author:
Annie Cavalier,Daniele Spehner,Bruno M. Humbel
Category:
Medicine
Subcat:
EPUB size:
1100 kb
FB2 size:
1568 kb
DJVU size:
1586 kb
Language:
Publisher:
CRC Press; 1 edition (October 2, 2008)
Pages:
706 pages
Rating:
4.4
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